Journal: Scientific Reports

Article Title: HDAC6, modulated by miR-206, promotes endometrial cancer progression through the PTEN/AKT/mTOR pathway

doi: 10.1038/s41598-020-60271-4

Figure Lengend Snippet: HDAC6 is upregulated in EC tissues and cell lines. ( A ) According to the Human Protein Atlas ( https://www.proteinatlas.org ), Kaplan-Meier survival curves indicated that high HDAC6 expression was correlated with poorer survival probability. ( B ) HDAC6 expression in different EC stages according to UALCAN ( http://ualcan.path.uab.edu/index.html ). ( C ) qRT-PCR showed higher HDAC6 expression in EC tissues than in paired adjacent normal tissues (n = 46). ( D ) Western blot analysis showed that cancer tissues expressed more HDAC6 than paracancerous control tissues (n = 46). ( E ) IHC analysis showed the HDAC6 expression levels in paraffin-embedded normal endometrial and EC specimens (100×, 200×). ( F ) Western blot analysis showed the HDAC6 expression levels in four selected EC cell lines—HEC-1-A, Ishikawa, AN3C, and RL95—and in one immortalized endometrial fibroblast cell line. ( G ) qRT-PCR showed HDAC6 mRNA levels in the EC cell lines and immortalized endometrial fibroblast cell line. ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The HEC-1-A and immortalized endometrial fibroblast cell lines were purchased from Icellbioscience (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Scientific Reports

Article Title: HDAC6, modulated by miR-206, promotes endometrial cancer progression through the PTEN/AKT/mTOR pathway

doi: 10.1038/s41598-020-60271-4

Figure Lengend Snippet: MiR-206 is downregulated in EC tissues and suppresses the proliferation, migration and invasion of endometrial cells in vitro . ( A ) qRT-PCR showed miR-206 levels in EC tissues (n = 46). ( B ) qRT-PCR showed miR-206 levels in the EC cell lines and the immortalized endometrial fibroblast cell line. ( C ) qRT-PCR showed miR-206 levels in Ishikawa and AN3C cells after transfection with generated lentiviruses. ( D ) CCK-8 assay of Ishikawa and AN3C cells transfected with the miR-206 inhibitor and miR-206 mimic. ( E ) Scratch wound assay of Ishikawa cells transfected with the miR-206 inhibitor and miR-206 mimic. Scale bar, 400 μm. ( F ) Transwell migration and invasion assays of Ishikawa cells after transfection with the miR-206 inhibitor and miR-206 mimic. Scale bar, 100 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The HEC-1-A and immortalized endometrial fibroblast cell lines were purchased from Icellbioscience (Shanghai, China).

Techniques: Migration, In Vitro, Quantitative RT-PCR, Transfection, Generated, CCK-8 Assay, Scratch Wound Assay Assay

Journal: Oncology Research

Article Title: Regulation of Histone Emulsification by HPDL via LDHA/LDHB Promotes EC Cell Proliferation

doi: 10.32604/or.2026.068833

Figure Lengend Snippet: Knockdown of HPDL decreased the level of histone lactonization modification in EC cells. ( A ) Western blot (WB) analysis confirming the expression of histone lactate dehydrogenase (HLD) antibodies in AN3CA cells with HPDL knockdown; ( B ) WB analysis confirming the expression of HLD antibodies in HEC-1-B cells with HPDL knockdown; ( C ) Quantitative polymerase chain reaction (qPCR) analysis of HPDL expression following triple-sequence plasmid knockdown in AN3CA cells; ( D ) qPCR analysis of HPDL expression following triple-sequence plasmid knockdown in HEC-1-B cells; ( E ) Measurement of lactate content in AN3CA cells subsequent to HPDL knockdown; ( F ) Measurement of lactate content in HEC-1-B cells subsequent to HPDL knockdown. ( G ) Green fluorescence signifies HPDL protein expression, while blue fluorescence indicates immunofluorescence staining of cell nuclei; scale bar is 50 μm; ( H ) Expression levels of HPDL, Pan Kla, and H3K18la after the addition of varying concentrations of sodium lactate to AN3CA Si HPDL knockdown cell lines; scale bar represents 50 μm. Expression levels of HPDL, Pan Kla, and H3K18la in AN3CA Si HPDL knockdown cell lines with the addition of varying concentrations of sodium lactate; scale bar represents 50 μm; ( I ) Expression levels of HPDL, Pan Kla, and H3K18la after the addition of varying concentrations of sodium lactate to AN3CA normal cell lines. *indicates p < 0.05, **indicates p < 0.01, ***indicates p < 0.001

Article Snippet: HEC-1-B cell line , Procell , CL-0100.

Techniques: Knockdown, Modification, Western Blot, Expressing, Real-time Polymerase Chain Reaction, Sequencing, Plasmid Preparation, Fluorescence, Immunofluorescence, Staining

Journal: Oncology Research

Article Title: Regulation of Histone Emulsification by HPDL via LDHA/LDHB Promotes EC Cell Proliferation

doi: 10.32604/or.2026.068833

Figure Lengend Snippet: The level of histone lactylation was elevated in EC cells overexpressing HPDL ( A ) Expression levels of HPDL, Pan Kla, and H3K18la following the overexpression of HPDL in the HEC-1-B cell line; ( B ) Expression levels of HPDL, Pan Kla, and H3K18la after treating the HEC-1-B cell LV-HPDL with varying concentrations of sodium oxalate; ( C ) Overexpression and knockdown strains of AN3CA; ( D ) Lactate levels in HEC-1-B overexpression and knockdown strains; ( E ) Immunofluorescence of AN3CA cells for quantifying H3K18la immunofluorescence across different groups; ( F ) Immunofluorescence of AN3CA cells for quantifying DAPI immunofluorescence across different groups; ( G ) Immunofluorescence of AN3CA cells for quantifying the expression of H3K18la and DAPI, scale bar is 50 μm; *indicating p < 0.05, **indicating p < 0.01, ***indicating p < 0.001, and ns indicating no statistical significance

Article Snippet: HEC-1-B cell line , Procell , CL-0100.

Techniques: Expressing, Over Expression, Knockdown, Immunofluorescence

Journal: Oncology Research

Article Title: Regulation of Histone Emulsification by HPDL via LDHA/LDHB Promotes EC Cell Proliferation

doi: 10.32604/or.2026.068833

Figure Lengend Snippet: The expression of HPDL promotes the proliferation of EC cells and affects the cell cycle. ( A ) CCK-8 profiles in AN3CA cells; ( B ) CCK-8 profiles in HEC-1-B cells; ( C ) Quantitative results of plate cloning assay of HEC-1-B cells; ( D ) Plate cloning assay of AN3CA and HEC-1-B cells; ( E ) Quantification results of plate cloning of AN3CA cells; ( F ) AN3CA cell cycle assay, *indicating p < 0.05 and ***indicating p < 0.001

Article Snippet: HEC-1-B cell line , Procell , CL-0100.

Techniques: Expressing, CCK-8 Assay, Cloning, Cell Cycle Assay

Journal: Oncology Research

Article Title: Regulation of Histone Emulsification by HPDL via LDHA/LDHB Promotes EC Cell Proliferation

doi: 10.32604/or.2026.068833

Figure Lengend Snippet: HPDL promotes the migration and invasion of EC cells. ( A ) Cell scratch assay of the normal, overexpression and knockdown groups of the HPDL gene in AN3CA cells, scale bar is 50 μm; ( B ) Cell scratch assay of the normal, overexpression and knockdown groups of the HPDL gene in HEC-1-B cells; ( C ) AN3CA and HEC-1-B cells with the normal, overexpression and knockdown groups of the HPDL gene in the quantitative histograms of cell scratch assay cells; ( D ) quantitative histograms of Transwell migration assay of AN3CA cells; ( E ) quantitative histograms of Transwell migration assay of HEC-1-B cells; ( F ) Transwell migration assay of AN3CA and HEC-1-B cells, scale bar is 50 μm; ( G ) AN3CA and HEC-1-B cells Transwell invasion assay; ( H ) AN3CA and HEC-1-B cell HPDL overexpression Transwell invasion assay; ( I ) Transwell invasion assay with HPDL knockdown in AN3CA and HEC-1-B cells, ***indicating p < 0.001

Article Snippet: HEC-1-B cell line , Procell , CL-0100.

Techniques: Migration, Wound Healing Assay, Over Expression, Knockdown, Transwell Migration Assay, Transwell Invasion Assay